Hands-On Proteomics Training

Hands-On Proteomics Workshop

Participants will learn introductory proteomics science and applicable protocols and technologies through a comprehensive, hands-on exploration of the various proteomics workflows. This course is appropriate for individuals with little to no experience in mass spectrometry and/or high performance liquid chromatography (HPLC) who desire a comprehensive overview of the typical proteomics workflows, including gel-based and on-line methods. Individuals who may primarily benefit from this course include: investigators who are considering incorporating proteomics protocols into their own research, graduate students and post-doctoral fellows, proteomics core users, industry sales representatives, bioinformaticists, and others who need to be familiar with typical proteomics workflows.

Lecture Topics: Introduction to Mass Spectrometry, Introduction to Database Searching, Front-End Technologies, Web Resources, Advanced Techniques, Spectral Interpretation, Clinical Proteomics, and Quantitative Proteomics. A Roundtable Discussion Session is also included.

Laboratories are divided into 3 stations where participants receive hands on experience in:

Sample Preparation: 1D gels, spot picking, in-gel digestion and cleanup, 2D gels (demonstration and mini-lecture), phosphopeptide enrichment, stains and visualization techniques (demonstration and mini-lecture)

Instrumentation: HPLC, Nano-LC/MS, Quadrupole-TOF, ESI-TOF, HPLC-Chip MS. Curriculum includes: hardware and software overview, calibration (TOFs), gradient design (LC methods), and hands-on experience on each instrument. Students analyze their own peptide digests using multiple instruments, which were accumulated during the Sample Preparation laboratories.

Practical Proteomics: Introduction to database searching, off-line fractionation techniques, and spectral interpretation.

Database Searching: The course culminates with a ½ day database searching laboratory where students analyze data they themselves accumulated during the course. Includes: Basic MS and MS/MS searching using MASCOT and Spectrum Mill, and spectral review and validation.

Dates and Registration